Plaquing procedure for infectious hematopoietic necrosis virus
نویسندگان
چکیده
منابع مشابه
Plaquing procedure for infectious hematopoietic necrosis virus.
A single overlay plaque assay was designed and evaluated for infectious hematopoietic necrosis virus. Epithelioma papillosum carpio cells were grown in normal atmosphere with tris(hydroxymethyl)aminomethane- or HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid)-buffered media. Plaques were larger and formed more quickly on 1- to 3-day-old cell monolayers than on older monolayers. Cell ...
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Infectious hematopoietic necrosis (IHN)-a highly lethal infectious salmonid disease-has caused substantial economic losses in the European production of rainbow trout (Oncorhynchus mykiss) since the late 1980s. The causal agent of IHN is the IHN virus (IHNV) introduced from overseas. However, until today, its phylogeographic spread in Europe remains poorly understood. We therefore sought to elu...
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This study investigated the stimulation of specific host defenses to IHNV that are created by prior exposure to an avirulent reovirus (chum salmon reovirus; CSV). Preexposure of rainbow trout to CSV for 1 h resulted in a relative percent survival that ranged from 68 to 100% when fish were challenged with IHNV over an 8 week period. The levels of serum neutralizing antibodies detected 28 d after...
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A nonrad~oac t~ve DNA probe assay was developed to detect and ~ d e n t ~ f y infect~ous hernatopoiet~c necrosls virus (IHNV) uslng a dot blot format The probe a synthet~c DNA oligonuc leot~de labeled enzymatlcally w ~ t h biotln hybnd~zed spec~f~cal ly w ~ t h nucleocaps~d mRNA extracted from Infected cells early In the vlrus repl~cation cycle A r a p ~ d g u a n ~ d l n ~ u m th~ocyanate base...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1980
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.39.4.872-876.1980